View article

Materials and Methods

Tempocholine (Molecule 11) Chloride. 7V, 7V-Dimethyl-A-(2', 2', 6', 6'-tetramethyl-4'-piperidyl)-2-acetoxyethylammonium bromide was prepared by the reaction of 4-(N, N-dimethylamino)-2, 2, 6, 6-tetramethylpiperidine with 2-bromoethyl acetate and then hydrolyzed, oxidized, and ion ex-changed to tempocholine bicarbonate. An aqueous solution of tempocholinebicarbonate was acidified with HC1 to yield tempocholine chloride. This procedure was partly due to Dr. Wayne L. Hubbell.(a) 4-((V,(V-Dimethylamino)-2, 2, 6, 6-tetramethylpiperidine was prepared by the method of Icke and Wisegarver (1955). 4-Amino-2, 2, 6, 6-tetramethylpiperidine(Aldrich Chemical Co.)(25 g) was added with magnetic stirring to 38 g of 98% formic acid in an ice bath. The mixture was removed from the ice bath, combined with 28.5 g of 37% formaldehyde, refluxed for about 10 hr, cooled in an ice bath, and com …